Microscopy: A Brief Overview

Microscopy is the scientific study of small objects using a microscope. It allows us to observe and analyze things that are too small to be seen with the naked eye.  

History of Microscopy

  • First Compound Microscope: Developed by Zacharias Janssen in 1595 in Holland.
  • Magnification: Initially ranged from 3X to 9X.

Key Terms in Microscopy

  • Magnification: The making of an object seem larger than it is.
  • Resolving Power (Resolution): The ability of a microscope to distinguish between two closely spaced objects.
    • Human eye can differentiate between two points at least 0.1 mm apart.

 

Understanding Magnification and Resolution

  • Magnification: Increases the size of an object, making it easier to see.  
  • Resolution: Determines the clarity of the image. A higher resolution provides greater clarity and precision.
  • Limitations: Even with high magnification, if the resolution is poor, the image will be blurry.
Note: To achieve both high magnification and high resolution, advanced microscopy techniques and equipment are often used, such as electron microscopes and confocal microscopes.